Augmenting the efficacy of anti-cocaine catalytic antibodies through chimeric hapten design and combinatorial vaccination.
Bioorg Med Chem Lett
; 27(16): 3666-3668, 2017 08 15.
Article
en En
| MEDLINE
| ID: mdl-28709828
ABSTRACT
Given the need for further improvements in anti-cocaine vaccination strategies, a chimeric hapten (GNET) was developed that combines chemically-stable structural features from steady-state haptens with the hydrolytic functionality present in transition-state mimetic haptens. Additionally, as a further investigation into the generation of an improved bifunctional antibody pool, sequential vaccination with steady-state and transition-state mimetic haptens was undertaken. While GNET induced the formation of catalytically-active antibodies, it did not improve overall behavioral efficacy. In contrast, the resulting pool of antibodies from GNE/GNT co-administration demonstrated intermediate efficacy as compared to antibodies developed from either hapten alone. Overall, improved antibody catalytic efficiency appears necessary to achieve the synergistic benefits of combining cocaine hydrolysis with peripheral sequestration.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Cocaína
/
Anticuerpos Catalíticos
/
Haptenos
Límite:
Animals
Idioma:
En
Año:
2017
Tipo del documento:
Article