Improved assay of the enzymes of glutathione synthesis: gamma-glutamylcysteine synthetase and glutathione synthetase.
Clin Chim Acta
; 158(1): 115-23, 1986 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-2874903
ABSTRACT
New methods for the estimation of red cell gamma-glutamylcysteine synthetase and glutathione synthetase have been developed. gamma-32P ATP is allowed to equilibrate until the gamma and beta phosphate groups are equally labelled. The amount of 32Pi released in the presence of glutamic acid and cysteine, the substrates for GC-S or in the presence of gamma-glutamylcysteine and glycine, the substrates of GSH-S, is measured. This is accomplished by extraction of the phosphomolybdate complex into isobutanol-benzene. The methods are linear with time and hemolysate concentration. Normal values are presented.
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Banco de datos:
MEDLINE
Asunto principal:
Péptido Sintasas
/
Glutamato-Cisteína Ligasa
/
Glutatión Sintasa
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Año:
1986
Tipo del documento:
Article