Secretogranin III promotes angiogenesis through MEK/ERK signaling pathway.
Biochem Biophys Res Commun
; 495(1): 781-786, 2018 01 01.
Article
en En
| MEDLINE
| ID: mdl-29154827
ABSTRACT
Secretogranin III (Scg3) was recently discovered as the first highly diabetic retinopathy-associated angiogenic factor, and its neutralizing antibody alleviated the disease with high efficacy in diabetic mice. Investigation of its molecular mechanisms will facilitate the translation of this novel therapy. Scg3 was reported to induce the phosphorylation of mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK). Here we characterized the importance of MEK/ERK activation to Scg3 angiogenic activity. Our results showed that MEK inhibitor PD98059 blocked Scg3-induced proliferation of human umbilical vein endothelial cells (HUVECs). This finding was corroborated by PD98059 inhibition of HUVEC migration and tube formation. Furthermore, ERK inhibitor SCH772984 also suppressed Scg3-induced proliferation and migration of HUVECs. Taken together, these findings suggest that MEK-ERK pathway plays an important role in Scg3-induced angiogenesis.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Cromograninas
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Sistema de Señalización de MAP Quinasas
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Células Endoteliales
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Proteínas Angiogénicas
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Neovascularización Patológica
Límite:
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article