Leucine and glycine dipeptides of porcine placenta ameliorate physical fatigue through enhancing dopaminergic systems.
Mol Med Rep
; 17(3): 4120-4130, 2018 03.
Article
en En
| MEDLINE
| ID: mdl-29286166
ABSTRACT
Fatigue is a common and serious health problem, and various dietary interventions have previously been employed to ameliorate fatigue. The aim of the current study was to investigate the antifatigue effects of Danish porcine placenta (DPP) and its major dipeptides, including leucineglycine (LG) and glycineleucine (GL). The antifatigue effects of orally administered DPP, LG and GL were determined using a treadmill exercise test and a forced swimming test (FST) in mice. Additionally, the antiinflammatory effects of DPP, LG and GL were investigated in activated splenocytes. The results demonstrated that oral treatment of mice with DPP, LG and GL increased the time to exhaustion during treadmill exercise. Furthermore, DPP, LG and GL enhanced the levels of dopamine, brainderived neurotrophic factor and phosphorylated-extracellular signalregulated kinase in the brains of mice with treadmill exerciseinduced exhaustive fatigue, and decreased levels of certain proinflammatory cytokines in the serum and spleen, as determined by ELISA and western blot analysis. Following treadmill exercise, commercial kits were employed to demonstrate that DPP, LG and GL reduced the levels of lactate dehydrogenase, lactate, creatine kinase, blood urea nitrogen, alanine transaminase and aspartate transaminase in the muscle and/or serum of mice. In addition, DPP, LG and GL enhanced the muscle and liver glycogen levels, catalase activity in the liver and serum superoxide dismutase activity. DPP, LG and GL also increased the proliferation of splenocytes and inhibited proinflammatory cytokine production by reducing the activation of caspase1 and nuclear factorκB in activated splenocytes, as determined by MTT assays, ELISA and western blotting, respectively. Furthermore, DPP, LG and GL reduced immobility time in the FST in mice. In conclusion, DPP may limit intensive exerciseinduced fatigue by increasing dopaminergic systems and inhibiting inflammatory responses.
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1
Banco de datos:
MEDLINE
Asunto principal:
Dopamina
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Agonistas de Dopamina
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Dipéptidos
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Fatiga
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Neuronas Dopaminérgicas
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Estimulantes del Sistema Nervioso Central
Límite:
Animals
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Pregnancy
Idioma:
En
Año:
2018
Tipo del documento:
Article