Efficient expression and isolation of recombinant human interleukin-11 (rhIL-11) in Pichia pastoris.

Yu, Kuo-Ming; Yiu-Nam Lau, Johnson; Fok, Manson; Yeung, Yuk-Keung; Fok, Siu-Ping; Shek, Felix; Wong, Wing-Tak; Choo, Qui-Lim

Yu, Kuo-Ming; Yiu-Nam Lau, Johnson; Fok, Manson; Yeung, Yuk-Keung; Fok, Siu-Ping; Shek, Felix; Wong, Wing-Tak; Choo, Qui-Lim.

Afiliación

Yu KM; Nansha Biologics (Hong Kong) Limited, Unit 608-613, IC Development Centre, No.6 Science Park West Avenue, Hong Kong Science Park, Shatin, Hong Kong Special Administrative Region, China; Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, 11 Yuk Choi Road, H
Yiu-Nam Lau J; Nansha Biologics (Hong Kong) Limited, Unit 608-613, IC Development Centre, No.6 Science Park West Avenue, Hong Kong Science Park, Shatin, Hong Kong Special Administrative Region, China.
Fok M; Nansha Biologics (Hong Kong) Limited, Unit 608-613, IC Development Centre, No.6 Science Park West Avenue, Hong Kong Science Park, Shatin, Hong Kong Special Administrative Region, China.
Yeung YK; Nansha Biologics (Hong Kong) Limited, Unit 608-613, IC Development Centre, No.6 Science Park West Avenue, Hong Kong Science Park, Shatin, Hong Kong Special Administrative Region, China.
Fok SP; Nansha Biologics (Hong Kong) Limited, Unit 608-613, IC Development Centre, No.6 Science Park West Avenue, Hong Kong Science Park, Shatin, Hong Kong Special Administrative Region, China.
Shek F; Nansha Biologics (Hong Kong) Limited, Unit 608-613, IC Development Centre, No.6 Science Park West Avenue, Hong Kong Science Park, Shatin, Hong Kong Special Administrative Region, China.
Wong WT; Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, 11 Yuk Choi Road, Hung Hom, Kowloon, Hong Kong.
Choo QL; Nansha Biologics (Hong Kong) Limited, Unit 608-613, IC Development Centre, No.6 Science Park West Avenue, Hong Kong Science Park, Shatin, Hong Kong Special Administrative Region, China.

Protein Expr Purif ; 146: 69-77, 2018 06.

Article en En | MEDLINE | ID: mdl-29408294

Asunto(s)

Interleucina-11/genética; Pichia/genética; Línea Celular; Proliferación Celular; Cromatografía en Gel; Clonación Molecular/métodos; Fermentación; Expresión Génica; Humanos; Interleucina-11/química; Interleucina-11/aislamiento & purificación; Interleucina-11/metabolismo; Pichia/metabolismo; Agregado de Proteínas; Replegamiento Proteico; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/aislamiento & purificación; Proteínas Recombinantes/metabolismo; Solubilidad

Palabras clave

IL-11; PEG; Secretory; Soluble aggregate; Yeast

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Pichia / Interleucina-11 Límite: Humans Idioma: En Año: 2018 Tipo del documento: Article

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Pichia / Interleucina-11 Límite: Humans Idioma: En Año: 2018 Tipo del documento: Article