Molecular and biological characterization of a highly pathogenic Trypanosoma cruzi strain isolated from a patient with congenital infection.
Exp Parasitol
; 186: 50-58, 2018 Mar.
Article
en En
| MEDLINE
| ID: mdl-29448038
ABSTRACT
Although many Trypanosoma cruzi (T. cruzi) strains isolated from a wide range of hosts have been characterized, there is a lack of information about biological features from vertically transmitted strains. We describe the molecular and biological characteristics of the T. cruzi VD strain isolated from a congenital Chagas disease patient. The VD strain was typified as DTU TcVI; in vitro sensitivity to nifurtimox (NFX) and beznidazole (BZ) were 2.88⯵M and 6.19⯵M respectively, while inhibitory concentrations for intracellular amastigotes were 0.24⯵M for BZ, and 0.66⯵M for NFX. Biological behavior of VD strain was studied in a mouse model of acute infection, resulting in high levels of parasitemia and mortality with a rapid clearence of bloodstream trypomastigotes when treated with BZ or NFX, preventing mortality and reducing parasitic load and intensity of inflammatory infiltrate in skeletal and cardiac muscle. Treatment-induced parasitological cure, evaluated after immunossupression were 41% and 35% for BZ and NFX treatment respectively, suggesting a partial response to these drugs in elimination of parasite burden. This exhaustive characterization of this T. cruzi strain provides the basis for inclusion of this strain in a panel of reference strains for drug screening and adds a new valuable tool for the study of experimental T. cruzi infection.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Tripanocidas
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Trypanosoma cruzi
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Enfermedad de Chagas
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Nifurtimox
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Nitroimidazoles
Tipo de estudio:
Clinical_trials
/
Prognostic_studies
Idioma:
En
Año:
2018
Tipo del documento:
Article