Transient Expression of AMPK Heterotrimer Complexes in Mammalian Cells.
Methods Mol Biol
; 1732: 159-169, 2018.
Article
en En
| MEDLINE
| ID: mdl-29480474
ABSTRACT
Regulation of AMP-activated protein kinase (AMPK) signalling is complex and involves contributions from adenine nucleotides, co-/posttranslational modifications, and isoform composition of the AMPK heterotrimer. It is becoming apparent that AMPK activation/inhibition by synthetic drugs involves similar levels of complexity. Major advances in our understanding of these mechanisms have been gained from recombinant expression systems that provide sufficient quantities of highly purified material for structure/function studies. Here, we provide a detailed protocol for transient expression of affinity-tagged AMPK complexes in mammalian cells. We have found this system to be optimal as a source of enzyme possessing regulatory modifications found in vivo.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Nucleótidos de Adenina
/
Subunidades de Proteína
/
Proteínas Quinasas Activadas por AMP
/
Pruebas de Enzimas
Límite:
Animals
/
Humans
Idioma:
En
Año:
2018
Tipo del documento:
Article