[Ligand-Induced Reassembly of GroEL/ES Chaperone In Vitro: Visualization by Electron Microscopy].
Mol Biol (Mosk)
; 52(1): 120-124, 2018.
Article
en Ru
| MEDLINE
| ID: mdl-29512644
ABSTRACT
The products of the reassembly reaction of tetradecameric two-ring quaternary structure of GroEL chaperonin under the pressure of its heptameric co-chaperonin GroES have been visualized by electron microscopy. It has been shown that one-ring heptameric oligomers of GroEL have been formed at the beginning (after ~5 min) of the reaction, while at the final stage of the reaction (after ~70 min), both one-ring heptamers in complex with one GroES and two-rings tetradecamers in complexes with one (asymmetrical complex) or two (symmetrical complex) GroES heptamers are present. The relationship between the data of light scattering, native electrophoresis, and electron microscopy obtained earlier has been discussed.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Chaperonina 60
/
Chaperonina 10
/
Proteínas de Escherichia coli
/
Escherichia coli
Idioma:
Ru
Año:
2018
Tipo del documento:
Article