[Swimming plus medication reduces the expressions of cytokines in rats with chronic abacterial prostatitis].

ABSTRACT

OBJECTIVE: To observe the effects of swimming plus medication on the expressions of cytokines in rats with chronic abacterial prostatitis (CAP). METHODS: Forty healthy adult male SD rats were randomly divided into five groups of equal number, normal control, CAP model control, medication, exercise therapy, and exercise + medication. The CAP model was made by Xiaozhiling injection, and at 7 days after modeling, the rats in the medication and exercise + medication groups were treated intragastrically with Qianlie Shutong Capsules (0.016 g/ml) at 20 ml per kg of the body weight qd, those in the exercise therapy and exercise + medication groups were made swim at a regular time once a day, 35 minutes on the first day and 5 minutes more on the second until 50 minutes once, for 4 successive weeks, and those in the normal control, model control and exercise therapy groups received normal saline only. After 14 and 28 days of treatment, all the rats were killed and their prostates harvested for observation of histopathological changes and determination of the expressions of TNF- α, IL-1ß and IL-6 in the prostatic tissue homogenate by ELISA. RESULTS: After 14 days of treatment, the expression levels of TNF-α, IL-1ß and IL-6 were significantly elevated in the groups of CAP model control (ï¼»183.08±8.07ï¼½ pg/ml, ï¼»57.55±3.53ï¼½ pg/ml and ï¼»256.15±13.95ï¼½ ng/L), medication (ï¼»118.49±8.06ï¼½ pg/ml, ï¼»42.64±4.64 ï¼½ pg/ml and ï¼»200.74±9.33ï¼½ ng/L), exercise therapy (ï¼»169.63±10.64ï¼½ pg/ml, ï¼»50.45±5.71ï¼½ pg/ml and ï¼»245.23±6.49ï¼½ ng/L), and exercise + medication (ï¼»107.82±7.81ï¼½ pg/ml, ï¼»40.35±6.93ï¼½ pg/ml and ï¼»187.04±10.85ï¼½ ng/L) as compared with those in the normal control (ï¼»20.36±1.82ï¼½ pg/ml, ï¼»14.64±1.91ï¼½ pg/ml and ï¼»70.58±2.09ï¼½ ng/L) (P<0.05). At 28 days, the levels of TNF- α, IL-1ß, IL-6 were remarkably lower in the exercise + medication group (ï¼»29.30±3.78ï¼½ pg/ml, ï¼»16.91±1.24ï¼½ pg/ml and ï¼» 88.65±6.74ï¼½ ng/L) than in the medication group (ï¼»39.67±3.19ï¼½ pg/ml, ï¼»26.27±3.49ï¼½ pg/ml and ï¼»110.26±6.33ï¼½ ng/L) (P<0.05) and close to those of the normal control group (ï¼»19.34±1.76ï¼½ pg/ml, ï¼»13.68±1.06ï¼½ pg/ml and ï¼»71.34±2.50ï¼½ ng/L). During the treatment, no obvious pathological changes were found in the prostate tissue of the normal control rats, while significant chronic prostatic inflammation was observed in the CAP models, and the inflammation was relieved in different degrees after intervention, most significantly in the exercise + medication group. CONCLUSIONS: Swimming can relieve prostatic inflammation and swimming plus medication can effectively reduce the expressions of cytokines and alleviate histological damage in the prostatic tissue of CAP rats.