Molecular diagnosis of Strongyloides stercoralis among transplant candidates.
Transpl Infect Dis
; 20(4): e12909, 2018 Aug.
Article
en En
| MEDLINE
| ID: mdl-29679429
ABSTRACT
Strongyloidiasis can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection, principally in immunosuppressed patients. Our study aimed to evaluate the application of conventional polymerase chain reaction (cPCR) and real-time PCR (qPCR). Polymerase chain reaction (PCR) and real-time PCR (qPCR) targeting the 18S rRNA gene for detection of Strongyloides stercoralis infection among transplant candidates were applied in stool samples obtained from 150 transplant candidates, preliminarily analyzed by parasitological methods. S. stercoralis larvae were visualized in 15/150 (10.0%) transplant candidates by parasitological methods. DNA from S. stercoralis was amplified in 26/150 (17.3%) and 49/150 (32.7%) stool samples of transplant candidates, using cPCR and qPCR, respectively. The results suggest that molecular methods, especially qPCR, should be used as an additional tool for diagnostic of S. stercoralis infection among transplant candidates.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Estrongiloidiasis
/
Strongyloides stercoralis
/
Análisis de Secuencia de ADN
/
ADN de Helmintos
/
Heces
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Prevalence_studies
/
Risk_factors_studies
Límite:
Animals
/
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Año:
2018
Tipo del documento:
Article