Measuring the bioactivity of anti-IL-6/anti-IL-6R therapeutic antibodies: presentation of a robust reporter gene assay.

Yu, Chuanfei; Cao, Junxia; Wang, Lan; Yang, Yalan; Ni, Yongbo; Wang, Junzhi

Yu, Chuanfei; Cao, Junxia; Wang, Lan; Yang, Yalan; Ni, Yongbo; Wang, Junzhi.

Afiliación

Yu C; Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Dongcheng District, Beijing, 100050, China.
Cao J; Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Dongcheng District, Beijing, 100050, China.
Wang L; Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Dongcheng District, Beijing, 100050, China.
Yang Y; Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Dongcheng District, Beijing, 100050, China.
Ni Y; Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Dongcheng District, Beijing, 100050, China.
Wang J; Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Dongcheng District, Beijing, 100050, China. wangjz_nifdc2014@163.com.

Anal Bioanal Chem ; 410(27): 7067-7075, 2018 Nov.

Article en En | MEDLINE | ID: mdl-30178083

Asunto(s)

Anticuerpos Monoclonales Humanizados/farmacología; Evaluación Preclínica de Medicamentos; Interleucina-6/antagonistas & inhibidores; Receptores de Interleucina-6/antagonistas & inhibidores; Anticuerpos Monoclonales Humanizados/inmunología; Ingeniería Celular; Línea Celular; Proliferación Celular; Evaluación Preclínica de Medicamentos/métodos; Genes Reporteros; Humanos; Interleucina-6/inmunología; Luciferasas/genética; Luciferasas/inmunología; Receptores de Interleucina-6/inmunología; Proteínas Recombinantes/inmunología

Palabras clave

Bioassay; IL-6; IL-6R; Reporter gene assay; Tocilizumab

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Interleucina-6 / Receptores de Interleucina-6 / Evaluación Preclínica de Medicamentos / Anticuerpos Monoclonales Humanizados Límite: Humans Idioma: En Año: 2018 Tipo del documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google