Melleolides from Honey Mushroom Inhibit 5-Lipoxygenase via Cys159.
Cell Chem Biol
; 26(1): 60-70.e4, 2019 01 17.
Article
en En
| MEDLINE
| ID: mdl-30415966
ABSTRACT
5-Lipoxygenase (5-LO) initiates the biosynthesis of pro-inflammatory leukotrienes from arachidonic acid, which requires the nuclear membrane-bound 5-LO-activating protein (FLAP) for substrate transfer. Here, we identified human 5-LO as a molecular target of melleolides from honey mushroom (Armillaria mellea). Melleolides inhibit 5-LO via an α,ß-unsaturated aldehyde serving as Michael acceptor for surface cysteines at the substrate entrance that are revealed as molecular determinants for 5-LO activity. Experiments with 5-LO mutants, where select cysteines had been replaced by serine, indicated that the investigated melleolides suppress 5-LO product formation via two distinct modes of action (1) by direct interference with 5-LO activity involving two or more of the cysteines 159, 300, 416, and 418, and (2) by preventing 5-LO/FLAP assemblies involving selectively Cys159 in 5-LO. Interestingly, replacement of Cys159 by serine prevented 5-LO/FLAP assemblies as well, implying Cys159 as determinant for 5-LO/FLAP complex formation at the nuclear membrane required for leukotriene biosynthesis.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Sesquiterpenos
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Araquidonato 5-Lipooxigenasa
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Inhibidores de la Lipooxigenasa
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Cisteína
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Armillaria
Límite:
Humans
Idioma:
En
Año:
2019
Tipo del documento:
Article