Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay.
Methods Mol Biol
; 1901: 95-102, 2019.
Article
en En
| MEDLINE
| ID: mdl-30539571
ABSTRACT
Agonistically acting autoantibodies directed against the adrenergic beta-1 receptor (beta1-AABs) are a pathogenic factor in diseases of the heart and circulatory system such as dilated cardiomyopathy. Here we describe the detection of such functionally active beta1-AABs from serum samples using spontaneously beating neonatal rat cardiomyocytes, which express the fully functional adrenergic beta-1 receptor coupled with the signal transduction pathway that induces chronotropy. With serum samples added (containing beta1-AABs), an increased positive chronotropic effect is caused that can be blocked by the subsequent addition of specific beta-blockers (abolishing this chronotropic response). The return to the basal beat rate of the cells by the addition of a beta-blocker proves the adrenergic beta-1 receptor specificity of the serum sample.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Autoanticuerpos
/
Bioensayo
/
Receptores Adrenérgicos beta 1
/
Antagonistas de Receptores Adrenérgicos beta 1
Límite:
Animals
Idioma:
En
Año:
2019
Tipo del documento:
Article