Differentiation of Plasmodium falciparum clones by means of a repetitive DNA probe.

Fucharoen, S; Tirawanchai, N; Wilairat, P; Panyim, S; Thaithong, S

Fucharoen, S; Tirawanchai, N; Wilairat, P; Panyim, S; Thaithong, S.

Afiliación

Fucharoen S; Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.

Trans R Soc Trop Med Hyg ; 82(2): 209-11, 1988.

Article en En | MEDLINE | ID: mdl-3055455

ABSTRACT

ABSTRACT

A recombinant DNA probe, pBRK1-14, was constructed by inserting a repetitive DNA fragment of 753 base pairs obtained from Plasmodium falciparum, isolate K1, into the EcoRI/PstI cloning sites of pBR322. This probe could discriminate between different parasite clones derived from a single isolate by hybridization with Southern genomic blots.

Asunto(s)

Clonación Molecular; Sondas de ADN; Plasmodium falciparum/genética; Animales; Autorradiografía; ADN Recombinante; Hibridación de Ácido Nucleico; Secuencias Repetitivas de Ácidos Nucleicos

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Banco de datos: MEDLINE Asunto principal: Plasmodium falciparum / Sondas de ADN / Clonación Molecular Límite: Animals Idioma: En Año: 1988 Tipo del documento: Article

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Imprimir

XML

PubMed Links

Banco de datos: MEDLINE Asunto principal: Plasmodium falciparum / Sondas de ADN / Clonación Molecular Límite: Animals Idioma: En Año: 1988 Tipo del documento: Article