Engineering synthetic artificial pancreas using chitosan hydrogels integrated with glucose-responsive microspheres for insulin delivery.
Mater Sci Eng C Mater Biol Appl
; 96: 374-382, 2019 Mar.
Article
en En
| MEDLINE
| ID: mdl-30606545
ABSTRACT
The closed-loop delivery of insulin in response to change of the blood glucose level and long-term supply of insulin are both important for diabetes patients. However, combination of these two goals in a chemically controlled implantable system is still challenging yet highly desirable. The purpose of the present study is to design a synthetic artificial pancreas by integration of chitosan hydrogels with insulin-loaded glucose-responsive microspheres to deliver insulin in a close-looped and long-term way for diabetes care. Glucose-responsive insulin-loaded microspheres were firstly fabricated via a high-speed shear-emulsion based crosslinking method and then embedded into chitosan hydrogels to make a scaffold-based synthetic artificial pancreas. In vitro experiments indicated the scaffold exhibited a longer insulin supply as well as a lower burst release compared with free microspheres, and could keep the glucose-responsive insulin release property inherited from the corresponding microspheres even after 12â¯day-release. The released insulin was proved to remain active, and the culture of HDF cells on the scaffold showed good cell proliferation during 7â¯days incubation. These results suggested the scaffold-based synthetic artificial pancreas have great promise in the application of insulin delivery.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Páncreas
/
Órganos Artificiales
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Hidrogeles
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Fibroblastos
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Insulina
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Microesferas
Límite:
Humans
Idioma:
En
Año:
2019
Tipo del documento:
Article