Chitosan coated calcium alginate beads for covalent immobilization of acrylamidase: Process parameters and removal of acrylamide from coffee.
Food Chem
; 275: 95-104, 2019 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-30724265
ABSTRACT
This study reports on removal of acrylamide from roasted coffee by acrylamidase from Cupriavidus oxalaticus ICTDB921. Chitosan coated calcium alginate beads were functionalized with citric acid as nontoxic cross linker and activated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) (1.661 w/w) for covalent immobilization of acrylamidase. The optimum beads were obtained using 5% sodium alginate, 1.5% chitosan, and 0.6â¯mol/L citric acid. The beads prepared at each step were characterized by FTIR and SEM. Coating of chitosan matrix on calcium alginate beads enhanced the mechanical stability over that of calcium alginate and/or chitosan. The immobilized acrylamidase showed optimum pH/temperature of 8.5/65⯰C, improved pH/thermal/shelf stability, and retained 80% activity after four cycles. Haldane model could describe the degradation kinetics of acrylamide in batch study. In packed bed column, a bed height, feed flow rate and inlet acrylamide concentration of 20â¯cm, 1â¯mL/min, and 100â¯mg/L gave best results.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Café
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Acrilamida
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Quitosano
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Alginatos
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Enzimas Inmovilizadas
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Manipulación de Alimentos
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Amidohidrolasas
Idioma:
En
Año:
2019
Tipo del documento:
Article