Novel Mussel-Inspired Universal Surface Functionalization Strategy: Protein-Based Coating with Residue-Specific Post-Translational Modification in Vivo.
ACS Appl Mater Interfaces
; 11(13): 12846-12853, 2019 Apr 03.
Article
en En
| MEDLINE
| ID: mdl-30843382
ABSTRACT
Surface functionalization can effectively endow materials with desirable properties, promoting the performance between the material and environment, with extensive applications. However, a universal and straightforward surface functionalization method with biocompatibility is scarce. In this study, with synthetic biology strategy, recombinant mussel plaque protein with a zwitterionic peptide inspired by molecular chaperone was engineered through post-translational modification, in which 3,4-dihydroxyphenylalanine was residue-specifically obtained efficiently from tyrosine with tyrosinase coexpressed in vivo. The rational designed chimeric protein coating in this work could successfully anchor to various substrates and exhibit excellent antifouling performance in resisting protein adsorption, cell attachment, and bacterial adhesion with eminent biocompatibility.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Dihidroxifenilalanina
/
Procesamiento Proteico-Postraduccional
/
Chaperonas Moleculares
/
Materiales Biocompatibles Revestidos
Límite:
Animals
Idioma:
En
Año:
2019
Tipo del documento:
Article