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Inkjet-printed micro-calibration standards for ultraquantitative Raman spectral cytometry.
LaLone, Vernon; Fawaz, Maria V; Morales-Mercado, Jomar; Mourão, Márcio A; Snyder, Catherine S; Kim, Sang Yeop; Lieberman, Andrew P; Tuteja, Anish; Mehta, Geeta; Standiford, Theodore J; Raghavendran, Krishnan; Shedden, Kerby; Schwendeman, Anna; Stringer, Kathleen A; Rosania, Gus R.
Afiliación
  • LaLone V; Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA. grosania@umich.edu.
  • Fawaz MV; Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.
  • Morales-Mercado J; School of Sciences, Technology, and Environment, Universidad Ana G. Méndez Cupey Campus, San Juan, Puerto Rico.
  • Mourão MA; CSCAR Center, University of Michigan, Ann Arbor, MI 48109, USA.
  • Snyder CS; Department of Materials Science and Engineering, College of Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
  • Kim SY; Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA. grosania@umich.edu.
  • Lieberman AP; Department of Pathology, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.
  • Tuteja A; Department of Materials Science and Engineering, College of Engineering, University of Michigan, Ann Arbor, MI 48109, USA and Biointerfaces Institute, Macromolecular Science and Engineering, Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
  • Mehta G; Department of Materials Science and Engineering, College of Engineering, University of Michigan, Ann Arbor, MI 48109, USA and Department of Biomedical Engineering, Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
  • Standiford TJ; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.
  • Raghavendran K; Department of Surgery, Michigan Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.
  • Shedden K; CSCAR Center, University of Michigan, Ann Arbor, MI 48109, USA.
  • Schwendeman A; Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA. grosania@umich.edu.
  • Stringer KA; Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109, USA and Department of Clinical Pharmacy, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.
  • Rosania GR; Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA. grosania@umich.edu.
Analyst ; 144(12): 3790-3799, 2019 Jun 21.
Article en En | MEDLINE | ID: mdl-31116195
ABSTRACT
Herein we report the development of a cytometric analysis platform for measuring the contents of individual cells in absolute (picogram) scales; this study represents the first report of Raman-based quantitation of the absolute mass - or the total amount - of multiple endogenous biomolecules within single-cells. To enable ultraquantitative calibration, we engineered single-cell-sized micro-calibration standards of known composition by inkjet-printer deposition of biomolecular components in microarrays across the surface of silicon chips. We demonstrate clinical feasibility by characterizing the compositional phenotype of human skin fibroblast and porcine alveolar macrophage cell populations in the respective contexts of Niemann-Pick disease and drug-induced phospholipidosis two types of lipid storage disorders. We envision this microanalytical platform as the foundation for many future biomedical applications, ranging from diagnostic assays to pathological analysis to advanced pharmaco/toxicokinetic research studies.
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Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Guideline / Prognostic_studies Idioma: En Año: 2019 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Guideline / Prognostic_studies Idioma: En Año: 2019 Tipo del documento: Article