An available strategy based on accurate mass by ultra high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry technology to characterization of metabolic profile of palbociclib in rat urine, feces and bile.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1124: 37-46, 2019 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-31176268
ABSTRACT
Palbociclib (named PD 0332991) is a novel highly selective cyclin-dependent kinase 4 and 6 (CDK 4/6) inhibitor, which has been approved by the Food and Drug Administration (FDA) for the treatment of hormone-receptor-positive advanced breast cancer. This present study developed a comprehensive strategy to investigate the metabolic profile of palbociclib in rat urine, feces and bile samples based on an ultra high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR MS). A total of 29 metabolites, including 18 phase I metabolites and 11 phase II metabolites, were detected and identified. The metabolic pathways included hydroxylation, oxidation, dehydrogenation, N-dealkylation, carbonylation, oxidative deamination, acetylation, glucuronidation, sulphate conjugation as well as the crossover of multiple metabolic pathways in vivo, and 16 of these metabolites were proposed for the first time. This study showed an insight into the metabolism of palbociclib in vivo, which may provide relevant chemical information for subsequent studies in the future.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Piperazinas
/
Piridinas
/
Espectrometría de Masas
/
Cromatografía Líquida de Alta Presión
/
Heces
/
Antineoplásicos
Tipo de estudio:
Evaluation_studies
Límite:
Animals
Idioma:
En
Año:
2019
Tipo del documento:
Article