Deregulation of H19 is associated with cervical carcinoma.
Genomics
; 112(1): 961-970, 2020 01.
Article
en En
| MEDLINE
| ID: mdl-31229557
ABSTRACT
CACX is one of the most common cancer affecting women world-wide. Here, expression microarray analysis revealed 8 over-expressed transcribed pseudogenes (GBP1P1, HLA-DRB6, HLA-H, SLC6A10P, NAPSB, KRT16P2, PTTG3P and RNF126P1), down-regulated 7 lincRNAs (H19, MIR100HG, MEG3, DIO3OS, HOXA11-AS, CD27-AS1 and EPB41L4A-AS) and 6 snoRNAs (SNORD97, SNORD3A, SNORD3C, SNORD3D, SNORA12 and SCARNA9) as DEncGs (log2 fold-changeâ¯≥⯱1.0) in CACX. Consequently, down-regulation of lincRNA MEG3 and over-expression of pseudogenes, GBP1P1 and PTTG3P in the microarray analysis were found concordant with the real-time quantitative PCR results upon validation. Then, Ingenuity® Pathway analysis (IPA®) analysis with deregulated DEncGs identified functionally important gene, H19. Further, validation (nâ¯=â¯52) of expression confirmed frequent downregulation of H19 with significant association with its deletion (LOH) and promoter methylation (nâ¯=â¯128) in CACX. Moreover, clinicopathological analysis found Indian CACX patients (nâ¯=â¯26) with alterations of H19 by deletion or, promoter methylation with concomitant low expression have poor prognosis.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Carcinoma
/
Neoplasias del Cuello Uterino
/
ARN Largo no Codificante
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Female
/
Humans
País/Región como asunto:
Asia
Idioma:
En
Año:
2020
Tipo del documento:
Article