A kinetic model to optimize and direct the dose ratio of Dsz enzymes in the 4S desulfurization pathway in vitro and in vivo.

ABSTRACT

OBJECTIVE: To enhance the biodesulfurization rate using a kinetic model that directs the ratio of Dsz enzymes. RESULTS: This study established a kinetic model that predicted the optimal ratio of Dsz enzymes in the 4S biodesulfurization system to be ABC = 124 and 142. When BCAD+1A+4B+2C, the conversion rate of dibenzothiophene (DBT) to 2-hydroxybiphenyl (HBP) was close to 100% in vitro. When the gene dose of dszC was increased, the HBP yield of the recombinant strain BL21(DE3)/BCAD + C reached approximately 0.012 mM in vivo, which was approximately 6-fold higher than that of the BCAD strain. CONCLUSIONS: According to the results predicted by the enzyme kinetic model, maintaining higher concentrations of DszC and DszB in the desulfurization system can effectively improve the desulfurization efficiency.