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A plant RNA virus hijacks endocytic proteins to establish its infection in plants.
Wu, Guanwei; Cui, Xiaoyan; Dai, Zhaoji; He, Rongrong; Li, Yinzi; Yu, Kangfu; Bernards, Mark; Chen, Xin; Wang, Aiming.
Afiliación
  • Wu G; London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford St, London, Ontario, N5V 4T3, Canada.
  • Cui X; Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, 210014, People's Republic of China.
  • Dai Z; Department of Biology, University of Western Ontario, 1151 Richmond ST, London, Ontario, N6A 5B7, Canada.
  • He R; Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, 210014, People's Republic of China.
  • Li Y; London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford St, London, Ontario, N5V 4T3, Canada.
  • Yu K; Department of Biology, University of Western Ontario, 1151 Richmond ST, London, Ontario, N6A 5B7, Canada.
  • Bernards M; London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford St, London, Ontario, N5V 4T3, Canada.
  • Chen X; Department of Biology, University of Western Ontario, 1151 Richmond ST, London, Ontario, N6A 5B7, Canada.
  • Wang A; London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford St, London, Ontario, N5V 4T3, Canada.
Plant J ; 101(2): 384-400, 2020 01.
Article en En | MEDLINE | ID: mdl-31562664
ABSTRACT
Endocytosis and endosomal trafficking play essential roles in diverse biological processes including responses to pathogen attack. It is well established that animal viruses enter host cells through receptor-mediated endocytosis for infection. However, the role of endocytosis in plant virus infection still largely remains unknown. Plant dynamin-related proteins 1 (DRP1) and 2 (DRP2) are the large, multidomain GTPases that participate together in endocytosis. Recently, we have discovered that DRP2 is co-opted by Turnip mosaic virus (TuMV) for infection in plants. We report here that DRP1 is also required for TuMV infection. We show that overexpression of DRP1 from Arabidopsis thaliana (AtDRP1A) promotes TuMV infection, and AtDRP1A interacts with several viral proteins including VPg and cylindrical inclusion (CI), which are the essential components of the virus replication complex (VRC). AtDRP1A colocalizes with the VRC in TuMV-infected cells. Transient expression of a dominant negative (DN) mutant of DRP1A disrupts DRP1-dependent endocytosis and supresses TuMV replication. As adaptor protein (AP) complexes mediate cargo selection for endocytosis, we further investigated the requirement of AP in TuMV infection. Our data suggest that the medium unit of the AP2 complex (AP2ß) is responsible for recognizing the viral proteins as cargoes for endocytosis, and knockout of AP2ß impairs intracellular endosomal trafficking of VPg and CI and inhibits TuMV replication. Collectively, our results demonstrate that DRP1 and AP2ß are two proviral host factors of TuMV and shed light into the involvement of endocytosis and endosomal trafficking in plant virus infection.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Virus de Plantas / Virus ARN / Proteínas Virales / Proteínas de Arabidopsis / Dinaminas Idioma: En Año: 2020 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Virus de Plantas / Virus ARN / Proteínas Virales / Proteínas de Arabidopsis / Dinaminas Idioma: En Año: 2020 Tipo del documento: Article