Single-cell assays using integrated continuous-flow microfluidics.
Methods Enzymol
; 628: 59-94, 2019.
Article
en En
| MEDLINE
| ID: mdl-31668236
ABSTRACT
The recent maturation of continuous-flow microfluidic technologies has coincided with transformative new methods to profile single cells, including their genetic types, protein expression and enzyme activities. Continuous-flow high-throughput single-cell screening and sorting can reveal relationships across cellular phenotypes (e.g., enzyme activity and secretion) and genetic fingerprints. This technology provides unique opportunities, as well as experimental and computational challenges, for integrative approaches that can process large amounts of single-cell data. In this chapter, we discuss recent advances in integrated continuous-flow microfluidic approaches with a focus on measurements and statistical analysis of single-cell enzyme activity and their applications in quantitative biology, synthetic biology, and diagnosis.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Analíticas Microfluídicas
/
Pruebas de Enzimas
/
Análisis de la Célula Individual
Límite:
Animals
/
Humans
Idioma:
En
Año:
2019
Tipo del documento:
Article