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Identification and partial purification of thuricin 4AJ1 produced by Bacillus thuringiensis.
Su, Xiaoyu; Li, Lifen; Pan, Jieru; Fan, Xiao; Ma, Shenglong; Guo, Yachong; Idris, Aisha Lawan; Zhang, Lingling; Pan, Xiaohong; Gelbic, Ivan; Huang, Tianpei; Guan, Xiong.
Afiliación
  • Su X; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
  • Li L; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
  • Pan J; Fuzhou Center for Disease Control and Prevention, Fuzhou, 350004, Fujian, China.
  • Fan X; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
  • Ma S; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
  • Guo Y; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
  • Idris AL; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
  • Zhang L; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
  • Pan X; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
  • Gelbic I; Biology Centre, Czech Academy of Sciences, Institute of Entomology, Branisovská 31, 370 05, Ceské Budejovice, Czech Republic. gelbic@entu.cas.cz.
  • Huang T; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. tianp
  • Guan X; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education, College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
Arch Microbiol ; 202(4): 755-763, 2020 May.
Article en En | MEDLINE | ID: mdl-31807807
ABSTRACT
Thuricin 4AJ1, produced by Bacillus thuringiensis strain 4AJ1, showed inhibition activity against Bacillus cereus 0938 and ATCC 10987. It began to appear in the stationary phase and reached its maximum activity level of 209.958 U at 18 h against B. cereus 0938 and 285.689 U at 24 h against B. cereus ATCC 10987. Tricine-SDS-PAGE results showed that the partly purified thuricin 4AJ1 was about 6.5 kDa. The molecular weights of the known B. thuringiensis bacteriocins and the ones obtained by the two mainstream websites for predicting bacteriocins were inconsistent with the size of the thuricin 4AJ1, indicating that the bacteriocin obtained in this study may have a novel structure. Based on the biochemical properties, the thuricin 4AJ1 activities increased after treatment with proteinase K and lipase II, and were not affected by a-amylase, catalase, α-chymotrypsin VII and α-chymotrypsin II. It was heat tolerant, being active up to 90º C. In the pH 3-10 range, it maintained most of its activity. Finally, the sensitivity of the strain 4AJ1 to commonly used antibiotics was tested. In view of its stability and antibacterial activity, thuricin 4AJ1 may be applied as a food biopreservative.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Bacillus thuringiensis / Bacteriocinas Tipo de estudio: Diagnostic_studies Idioma: En Año: 2020 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Bacillus thuringiensis / Bacteriocinas Tipo de estudio: Diagnostic_studies Idioma: En Año: 2020 Tipo del documento: Article