Novel screening procedure for recombinant plasmids.

Telford, J; Boseley, P; Schaffner, W; Birnstiel, M

Telford, J; Boseley, P; Schaffner, W; Birnstiel, M.

Science ; 195(4276): 391-3, 1977 Jan 28.

Article en En | MEDLINE | ID: mdl-318763

ABSTRACT

ABSTRACT

Lysed bacterial colonies containing potential recombinant plasmids were mixed with molten agar and sealed into slots of an agarose gel. After electrophoresis overnight, the gel was stained with ethidium bromide, which clearly reveals recombinant plasmids. Xenopus laevis ribosomal DNA and histone DNA of Psammechinus miliaris were ligated into pCRI plasmids and screened by this method.

Asunto(s)

ADN Bacteriano/análisis; ADN Recombinante; Herencia Extracromosómica; Plásmidos; Recombinación Genética; Animales; Equinodermos; Electroforesis en Gel de Agar; Escherichia coli; Genes; Ingeniería Genética; Histonas; Métodos; ARN Ribosómico; Xenopus

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Banco de datos: MEDLINE Asunto principal: Plásmidos / Recombinación Genética / ADN Bacteriano / ADN Recombinante / Herencia Extracromosómica Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals Idioma: En Año: 1977 Tipo del documento: Article

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