Development and Validation of HPLC Method for Determination of Sodium Copper Chlorophyllin - A Food Colorant and Its Application in Pharmacokinetic Study.
Chem Biodivers
; 17(8): e2000223, 2020 Aug.
Article
en En
| MEDLINE
| ID: mdl-32662938
ABSTRACT
A simple, accurate, and precise bioanalytical method was developed and validated for the determination of pharmacokinetic parameters of sodium copper chlorophyllin, a USFDA approved food additive and colorant in rat plasma. The column used was Luna® C18 250×4.6â
mm, 100â
Å, having particle size 4.5 µm, and the mobile phase used was methanol (MeOH), and 10â
mM ammonium acetate buffer in the ratio of 90 10, the flow rate was 1â
ml/min, and the injection volume of 20â
µL. The retention time of sodium copper chlorophyllin was obtained at 9â
min. The method was found to be linear at the range of 0.50-8.00â
µg mL-1 .
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Texto completo:
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Banco de datos:
MEDLINE
Asunto principal:
Clorofilidas
/
Cromatografía Líquida de Alta Presión
/
Colorantes de Alimentos
Límite:
Animals
Idioma:
En
Año:
2020
Tipo del documento:
Article