Structural basis for diamide modulation of ryanodine receptor.
Nat Chem Biol
; 16(11): 1246-1254, 2020 11.
Article
en En
| MEDLINE
| ID: mdl-32807966
ABSTRACT
The diamide insecticide class is one of the top-selling insecticides globally. They are used to control a wide range of pests by targeting their ryanodine receptors (RyRs). Here, we report the highest-resolution cryo-electron microscopy (cryo-EM) structure of RyR1 in the open state, in complex with the anthranilic diamide chlorantraniliprole (CHL). The 3.2-Å local resolution map facilitates unambiguous assignment of the CHL binding site. The molecule induces a conformational change by affecting the S4-S5 linker, triggering channel opening. The binding site is further corroborated by mutagenesis data, which reveal how diamide insecticides are selective to the Lepidoptera group of insects over honeybee or mammalian RyRs. Our data reveal that several pests have developed resistance via two mechanisms, steric hindrance and loss of contact. Our results provide a foundation for the development of highly selective pesticides aimed at overcoming resistance and therapeutic molecules to treat human myopathies.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Bloqueadores de los Canales de Calcio
/
Canal Liberador de Calcio Receptor de Rianodina
/
Diamida
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Ortoaminobenzoatos
/
Insecticidas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2020
Tipo del documento:
Article