CRISPRoff enables spatio-temporal control of CRISPR editing.
Nat Commun
; 11(1): 5041, 2020 10 07.
Article
en En
| MEDLINE
| ID: mdl-33028827
ABSTRACT
Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
ARN Guía de Kinetoplastida
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Estabilidad del ARN
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Sistemas CRISPR-Cas
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Edición Génica
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Luz
Límite:
Humans
Idioma:
En
Año:
2020
Tipo del documento:
Article