Prophylactic and therapeutic supplementation using fructo-oligosaccharide improves the intestinal homeostasis after mucositis induced by 5- fluorouracil.
Biomed Pharmacother
; 133: 111012, 2021 Jan.
Article
en En
| MEDLINE
| ID: mdl-33254017
ABSTRACT
The beneficial effects of prebiotic, such as fructo-oligosaccharides (FOS), in intestinal inflammation have been demonstrated in several studies. Herein, we evaluate whether joint treatment with FOS, both before and during mucositis, had additional beneficial effects and investigated the mechanisms underlying in the action of FOS on the intestinal barrier. BALB/c mice were randomly divided into five groups CTR (without mucositisâ¯+â¯saline solution), FOS (without mucositisâ¯+â¯6 % FOS), MUC (mucositisâ¯+â¯saline solution), PT (mucositisâ¯+â¯6 % FOS supplementation before disease induction), and TT (mucositisâ¯+â¯6 % FOS supplementation before and during disease induction). Mucositis was induced by intraperitoneal injection (300â¯mg/kg) of 5-fluorouracil (5-FU). After 72â¯h, the animals were euthanized and intestinal permeability (IP), tight junction, bacterial translocation (BT), histology and morphometry, and immunoglobulin A secretory (sIgA), inflammatory infiltrate, and production of short-chain fatty acids (acetate, butyrate and propionate) were evaluated. The MUC group showed an increase in the IP, BT, and inflammatory infiltrate but a decrease in the tight junction expression and butyrate and propionate levels (Pâ¯<â¯0.05). In the PT and TT groups, FOS supplementation maintained the IP, tight junction expression, and propionate concentration within physiologic levels, increased butyrate levels, and reduced BT and inflammatory infiltrate (Pâ¯<â¯0.05). Total treatment with FOS (TT group) was more effective in maintaining histological score, morphometric parameters, and sIgA production. Thus, total treatment (prophylactic and therapeutic supplementation) with FOS was more effective than pretreatment alone, in reducing 5-FU-induced damage to the intestinal barrier.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Oligosacáridos
/
Bacterias
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Uniones Estrechas
/
Mucositis
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Ácidos Grasos Volátiles
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Prebióticos
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Microbioma Gastrointestinal
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Íleon
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Mucosa Intestinal
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2021
Tipo del documento:
Article