Multicolor FRET-FLIM Microscopy to Analyze Multiprotein Interactions in Live Cells.

Ahmed, Abdullah; Schoberer, Jennifer; Cooke, Emily; Botchway, Stanley W

Ahmed, Abdullah; Schoberer, Jennifer; Cooke, Emily; Botchway, Stanley W.

Afiliación

Ahmed A; Central Laser Facility, Science and Technology Facilities Council (STFC) Rutherford Appleton Laboratory, Research Complex at Harwell, Oxford, UK.
Schoberer J; Plant Cell Biology, Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.
Cooke E; Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, BOKU Vienna, Vienna, Austria.
Botchway SW; Central Laser Facility, Science and Technology Facilities Council (STFC) Rutherford Appleton Laboratory, Research Complex at Harwell, Oxford, UK.

Methods Mol Biol ; 2247: 287-301, 2021.

Article en En | MEDLINE | ID: mdl-33301124

Asunto(s)

Transferencia Resonante de Energía de Fluorescencia/métodos; Microscopía Fluorescente/métodos; Imagen Molecular/métodos; Complejos Multiproteicos/metabolismo; Algoritmos; ADN/química; Modelos Teóricos; Unión Proteica

Palabras clave

Confocal microscopy; DNA; Enzymes; Excited-state lifetime; FLIM GFP-tag; FRET; Fluorescence imaging; Protein complex; TCSPC; Three-color; mTOR

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Transferencia Resonante de Energía de Fluorescencia / Complejos Multiproteicos / Imagen Molecular / Microscopía Fluorescente Idioma: En Año: 2021 Tipo del documento: Article

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