Isolation of autophagic fractions from mouse liver for biochemical analyses.
STAR Protoc
; 2(3): 100730, 2021 09 17.
Article
en En
| MEDLINE
| ID: mdl-34430907
ABSTRACT
Isolation of autophagosomes, autolysosomes, and lysosomes allows mechanistic studies into the pathophysiology of autophagy-a lysosomal quality control pathway. Here, we outline a Nycodenz density gradient ultracentrifugation approach for high-yield isolation of autophagic fractions from mouse liver. These fractions can be used for immunoblotting, transmission electron microscopy, and proteomic and lipidomic analyses. For complete details on the use and execution of this protocol, please refer to Toledo et al. (2018).
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Extractos Celulares
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Centrifugación por Gradiente de Densidad
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Autofagosomas
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Hígado
/
Lisosomas
Límite:
Animals
Idioma:
En
Año:
2021
Tipo del documento:
Article