Self-calcifying lipid nanocarrier for bone tissue engineering.
Biochim Biophys Acta Gen Subj
; 1866(2): 130047, 2022 02.
Article
en En
| MEDLINE
| ID: mdl-34757163
ABSTRACT
BACKGROUND:
A nanoemulsion with specific surface properties (such as charge and functional groups) can initiate the deposition of calcium phosphate (CaP) on its surface, leading to formation of CaP nanoparticles with a lipid core. The lipid core can carry lipophilic compounds based on the function of the nanoemulsion. Therefore, a dual purpose nanoemulsion of lipid nanoparticles (LNPs) exhibiting self-calcifying and carrier abilities can be developed.METHODS:
We employed an emulsification process to formulate LNPs with a specific charged surface. The LNPs were tested for their ability to calcify in simulated body fluid and encapsulate cholecalciferol (a model of active compound). The self-calcifying LNP was successfully fabricated using the emulsification process and stabilized using a mixture of polysorbate 80 and polysorbate 20.RESULTS:
The LNPs incubated in simulated body fluid bound to calcium and phosphate, subsequently forming CaP on the particle surface and resulting in approximately 180-nm CaP spheres with a lipid core. The LNPs facilitated calcium phosphate deposition in the collagen scaffolds. In addition, LNPs can be used as carriers of lipophilic compounds without impeding the self-calcifying ability.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ingeniería de Tejidos
Idioma:
En
Año:
2022
Tipo del documento:
Article