Site-specific proteomic strategies to identify ubiquitin and SUMO modifications: Challenges and opportunities.
Semin Cell Dev Biol
; 132: 97-108, 2022 12.
Article
en En
| MEDLINE
| ID: mdl-34802913
ABSTRACT
Ubiquitin and SUMO modify thousands of substrates to regulate most cellular processes. System-wide identification of ubiquitin and SUMO substrates provides global understanding of their cellular functions. In this review, we discuss the biological importance of site-specific modifications by ubiquitin and SUMO regulating the DNA damage response, protein quality control and cell cycle progression. Furthermore we discuss the machinery responsible for these modifications and methods to purify and identify ubiquitin and SUMO modified sites by mass spectrometry. We provide a framework to aid in the selection of appropriate purification, digestion and acquisition strategies suited to answer different biological questions. We highlight opportunities in the field for employing innovative technologies, as well as discuss challenges and long-standing questions in the field that are difficult to address with the currently available tools, emphasizing the need for further innovation.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ubiquitina
/
Proteómica
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2022
Tipo del documento:
Article