Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus.
Methods Mol Biol
; 2433: 227-235, 2022.
Article
en En
| MEDLINE
| ID: mdl-34985748
ABSTRACT
Liposomal encapsulation serves as the basis for the engineering of biomimetic and novel synthetic cells. Liposomes are normally formed using such methods as thin film rehydration (TFH), density-mediated reverse emulsion encapsulation (REE), or one of many microfluidics-based approaches-with the latter of these two methods being used mainly for the encapsulation of various lumen constituents such as cell-free protein expression reactions. Here, we describe the simultaneous formation and encapsulation of liposomes and various cell-mimetic lumen chemistries, respectively, using a 3D-printable microcapillary-based microfluidics device based off of the droplet-shooting and size-filtration (DSSF) liposome preparation method.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Microfluídica
/
Liposomas
Idioma:
En
Año:
2022
Tipo del documento:
Article