Your browser doesn't support javascript.
loading
Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.
Cui, Zhaokang; Lu, Yajuan; Miao, Yilong; Dai, Xiaoxin; Zhang, Yu; Xiong, Bo.
Afiliación
  • Cui Z; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
  • Lu Y; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
  • Miao Y; Institute of Reproductive Medicine, School of Medicine, Nantong University, Nantong, 226019, China.
  • Dai X; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
  • Zhang Y; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
  • Xiong B; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Cell Death Differ ; 29(8): 1466-1473, 2022 08.
Article en En | MEDLINE | ID: mdl-35017645
ABSTRACT
Soon after fertilization, the block mechanisms are developed in the zona pellucida (ZP) and plasma membrane of the egg to prevent any additional sperm from binding, penetration, and fusion. However, the molecular basis and underlying mechanism for the post-fertilization block to sperm penetration through ZP has not yet been determined. Here, we find that transglutaminase 2 (Tgm2), an enzyme that catalyzes proteins by the formation of an isopeptide bond within or between polypeptide chains, crosslinks zona pellucida glycoprotein 3 (ZP3) to result in the ZP hardening after fertilization and thus prevents polyspermy. Tgm2 abundantly accumulates in the subcortical region of the oocytes and vanishes upon fertilization. Both inhibition of Tgm2 activity in oocytes by the specific inhibitor in vitro and genetic ablation of Tgm2 in vivo cause the presence of additional sperm in the perivitelline space of fertilized eggs, consequently leading to the polyploid embryos. Biochemically, recombinant Tgm2 binds to and crosslinks ZP3 proteins in vitro, and incubation of oocytes with recombinant Tgm2 protein inhibits the polyspermy. Altogether, our data identify Tgm2 as a participant of zona block to the post-fertilization sperm penetration via hardening ZP surrounding fertilized eggs, extending our current understanding about the molecular basis of block to polyspermy.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Semen / Glicoproteínas de la Zona Pelúcida / Proteína Glutamina Gamma Glutamiltransferasa 2 Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Semen / Glicoproteínas de la Zona Pelúcida / Proteína Glutamina Gamma Glutamiltransferasa 2 Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article