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The Serological Sciences Network (SeroNet) for COVID-19: Depth and Breadth of Serology Assays and Plans for Assay Harmonization.
Karger, Amy B; Brien, James D; Christen, Jayne M; Dhakal, Santosh; Kemp, Troy J; Klein, Sabra L; Pinto, Ligia A; Premkumar, Lakshmanane; Roback, John D; Binder, Raquel A; Boehme, Karl W; Boppana, Suresh; Cordon-Cardo, Carlos; Crawford, James M; Daiss, John L; Dupuis, Alan P; Espino, Ana M; Firpo-Betancourt, Adolfo; Forconi, Catherine; Forrest, J Craig; Girardin, Roxie C; Granger, Douglas A; Granger, Steve W; Haddad, Natalie S; Heaney, Christopher D; Hunt, Danielle T; Kennedy, Joshua L; King, Christopher L; Krammer, Florian; Kruczynski, Kate; LaBaer, Joshua; Lee, F Eun-Hyung; Lee, William T; Liu, Shan-Lu; Lozanski, Gerard; Lucas, Todd; Mendu, Damodara Rao; Moormann, Ann M; Murugan, Vel; Okoye, Nkemakonam C; Pantoja, Petraleigh; Payne, Anne F; Park, Jin; Pinninti, Swetha; Pinto, Amelia K; Pisanic, Nora; Qiu, Ji; Sariol, Carlos A; Simon, Viviana; Song, Lusheng.
Afiliación
  • Karger AB; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota.
  • Brien JD; Department of Molecular Microbiology & Immunology, Saint Louis University, Saint Louis, Missouri.
  • Christen JM; Frederick National Laboratory for Cancer Research, Frederick, Maryland.
  • Dhakal S; W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Kemp TJ; Frederick National Laboratory for Cancer Research, Frederick, Maryland.
  • Klein SL; W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Pinto LA; Frederick National Laboratory for Cancer Research, Frederick, Maryland.
  • Premkumar L; Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC.
  • Roback JD; Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia.
  • Binder RA; Department of Medicine, University of Massachusetts Chan Medical School, Worcester, Massachusetts.
  • Boehme KW; Department of Microbiology & Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
  • Boppana S; Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama.
  • Cordon-Cardo C; Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama.
  • Crawford JM; Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Daiss JL; Department of Pathology and Laboratory Medicine, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York.
  • Dupuis AP; MicroB-plex, Inc., Atlanta, Georgia.
  • Espino AM; Wadsworth Center, New York State Department of Health, Albany, New York.
  • Firpo-Betancourt A; Department of Microbiology and Medical Zoology, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico.
  • Forconi C; Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Forrest JC; Department of Medicine, University of Massachusetts Chan Medical School, Worcester, Massachusetts.
  • Girardin RC; Department of Microbiology & Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
  • Granger DA; Wadsworth Center, New York State Department of Health, Albany, New York.
  • Granger SW; Salimetrics, LLC, Carlsbad, California.
  • Haddad NS; Salimetrics, LLC, Carlsbad, California.
  • Heaney CD; Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, Emory University, Atlanta, Georgia.
  • Hunt DT; Department of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Kennedy JL; Wadsworth Center, New York State Department of Health, Albany, New York.
  • King CL; Departments of Pediatrics and Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
  • Krammer F; Arkansas Children's Research Institute, Little Rock, Arkansas.
  • Kruczynski K; Department of Pathology, Case Western Reserve School of Medicine, Cleveland, Ohio.
  • LaBaer J; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Lee FE; Department of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Lee WT; Virginia G Piper Center for Personalized Diagnostics, Arizona State University Biodesign Institute, Tempe, Arizona.
  • Liu SL; Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, Emory University, Atlanta, Georgia.
  • Lozanski G; Wadsworth Center, New York State Department of Health, Albany, New York.
  • Lucas T; Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, New York.
  • Mendu DR; Center for Retrovirus Research, Department of Veterinary Biosciences, Department of Microbial Infection and Immunity, Viruses and Emerging Pathogens Program, Infectious Disease Institute, The Ohio State University, Columbus, Ohio.
  • Moormann AM; Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio.
  • Murugan V; Division of Public Health and Department of Epidemiology, College of Human Medicine, Michigan State University, East Lansing, Michigan.
  • Okoye NC; Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York.
  • Pantoja P; Department of Medicine, University of Massachusetts Chan Medical School, Worcester, Massachusetts.
  • Payne AF; Virginia G Piper Center for Personalized Diagnostics, Arizona State University Biodesign Institute, Tempe, Arizona.
  • Park J; Department of Pathology and Laboratory Medicine, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York.
  • Pinninti S; Unit of Comparative Medicine, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico.
  • Pinto AK; Wadsworth Center, New York State Department of Health, Albany, New York.
  • Pisanic N; Virginia G Piper Center for Personalized Diagnostics, Arizona State University Biodesign Institute, Tempe, Arizona.
  • Qiu J; Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama.
  • Sariol CA; Department of Molecular Microbiology & Immunology, Saint Louis University, Saint Louis, Missouri.
  • Simon V; Department of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.
  • Song L; Virginia G Piper Center for Personalized Diagnostics, Arizona State University Biodesign Institute, Tempe, Arizona.
medRxiv ; 2022 Mar 14.
Article en En | MEDLINE | ID: mdl-35262095
ABSTRACT

Background:

In October 2020, the National Cancer Institute (NCI) Serological Sciences Network (SeroNet) was established to study the immune response to COVID-19, and "to develop, validate, improve, and implement serological testing and associated technologies." SeroNet is comprised of 25 participating research institutions partnering with the Frederick National Laboratory for Cancer Research (FNLCR) and the SeroNet Coordinating Center. Since its inception, SeroNet has supported collaborative development and sharing of COVID-19 serological assay procedures and has set forth plans for assay harmonization.

Methods:

To facilitate collaboration and procedure sharing, a detailed survey was sent to collate comprehensive assay details and performance metrics on COVID-19 serological assays within SeroNet. In addition, FNLCR established a protocol to calibrate SeroNet serological assays to reference standards, such as the U.S. SARS-CoV-2 serology standard reference material and First WHO International Standard (IS) for anti-SARS-CoV-2 immunoglobulin (20/136), to facilitate harmonization of assay reporting units and cross-comparison of study data.

Results:

SeroNet institutions reported development of a total of 27 ELISA methods, 13 multiplex assays, 9 neutralization assays, and use of 12 different commercial serological methods. FNLCR developed a standardized protocol for SeroNet institutions to calibrate these diverse serological assays to reference standards.

Conclusions:

SeroNet institutions have established a diverse array of COVID-19 serological assays to study the immune response to SARS-CoV-2 virus and vaccines. Calibration of SeroNet serological assays to harmonize results reporting will facilitate future pooled data analyses and study cross-comparisons.
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2022 Tipo del documento: Article
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2022 Tipo del documento: Article