EpsRAc is a copper-sensing MarR family transcriptional repressor from Acidithiobacillus caldus.
Appl Microbiol Biotechnol
; 106(9-10): 3679-3689, 2022 May.
Article
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| MEDLINE
| ID: mdl-35583698
ABSTRACT
The MarR family, as multiple antibiotic resistance regulators, is associated with the resistance of organisms to unfavorable conditions. MarR family extracellular polymeric substances (EPS)-associated transcriptional regulator (EpsRAc) was closely associated with copper resistance in Acidithiobacillus caldus (A. caldus). Transcriptional analysis showed high activity of the epsR promoter (PI) in Escherichia coli and differential response to metal ions. The copper content and UV absorption spectrum of the co-purified protein did not increase, but a stoichiometry of 0.667 mol Cu(I) per EpsRAc monomer was observed in vitro in copper titration experiments, suggesting that Cu(II) acts with low affinity in binding to the EpsRAc protein. Electrophoretic mobility shift assays (EMSA) demonstrated that EpsRAc could bind to its own promoter in vitro, and the binding region was the palindrome sequence TGTTCATCGTGTGTGAGCACACA. EpsRAc negatively regulated its own gene expression, whereas Cu(II) mitigates this negative effect. EpsRAc did not bind to other neighboring gene promoters. Finally, we developed a working model to illustrate the regulatory mechanism of A. caldus in response to extreme copper stress. KEY POINTS ⢠Identification of a MarR family EPS-associated transcriptional regulator, named EpsRAc. ⢠Cu(I) can bind to the EpsRAc protein with low affinity. ⢠EpsRAc negatively regulates the expression of epsR, and Cu(II) can alleviate this negative regulation.
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MEDLINE
Asunto principal:
Proteínas de Escherichia coli
/
Acidithiobacillus
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2022
Tipo del documento:
Article