Impact of overexpressing NADH kinase on glucoamylase production in Aspergillus niger.
J Ind Microbiol Biotechnol
; 49(4)2022 Jul 30.
Article
en En
| MEDLINE
| ID: mdl-35665816
ABSTRACT
Glucoamylase has a wide range of applications in the production of glucose, antibiotics, amino acids, and other fermentation industries. Fungal glucoamylase, in particular, has attracted much attention because of its wide application in different industries, among which Aspergillus niger is the most popular strain producing glucoamylase. The low availability of NADPH was found to be one of the limiting factors for the overproduction of glucoamylase. In this study, 3 NADH kinases (AN03, AN14, and AN17) and malic enzyme (maeA) were overexpressed in aconidial A. niger by CRISPR/Cas9 technology, significantly increasing the size of the NADPH pool, resulting in the activity of glucoamylase was improved by about 70%, 50%, 90%, and 70%, respectively; the total secreted protein was increased by about 25%, 22%, 52%, and 26%, respectively. Furthermore, the combination of the mitochondrial NADH kinase (AN17) and the malic enzyme (maeA) increased glucoamylase activity by a further 19%. This study provided an effective strategy for enhancing glucoamylase production of A. niger.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Aspergillus niger
/
Glucano 1,4-alfa-Glucosidasa
Idioma:
En
Año:
2022
Tipo del documento:
Article