High-level, heat-regulated synthesis of proteins in eukaryotic cells.
Gene
; 49(1): 1-8, 1986.
Article
en En
| MEDLINE
| ID: mdl-3569912
ABSTRACT
Plasmids have been constructed in which promoters of 70-kDa heat-shock protein genes (hsp70) of human and Drosophila origin were linked to three different eukaryotic genes encoding human growth hormone (hGH), chicken lysozyme (cL) and a human influenza haemagglutinin (HA). Following transfection into widely divergent eukaryotic cells, the hybrid genes direct the transient, heat-regulated synthesis of the three proteins. hGH and cL are secreted into the medium. A human hsp70-hGH construct was used to establish stable mouse fibroblast lines that are capable of producing and secreting hGH at high levels following heat induction hGH is secreted at a 500-1200-fold higher rate by heat-treated than by untreated cells.
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Banco de datos:
MEDLINE
Asunto principal:
Genes
/
Proteínas de Choque Térmico
/
Ligamiento Genético
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Año:
1986
Tipo del documento:
Article