Rapid screening assay for phospholipase C activity in mycoplasmas.
J Clin Microbiol
; 25(4): 729-31, 1987 Apr.
Article
en En
| MEDLINE
| ID: mdl-3571480
ABSTRACT
A screening assay for phospholipase C using a chromogenic substrate incorporated into agar medium is described. The assay directly visualizes phospholipase C activity of mycoplasma lysates and membranes on agar plates, or the activity may be measured by spectrophotometry. The results from the assay confirm the presence in Ureaplasma urealyticum of phospholipase C, which is predominantly localized in the membrane fraction. The procedure has the potential to screen phospholipase C activity in other mycoplasmas and microorganisms in general.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fosfolipasas de Tipo C
/
Ureaplasma
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Año:
1987
Tipo del documento:
Article