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Probing Neuropeptide Volume Transmission In Vivo by Simultaneous Near-Infrared Light-Triggered Release and Optical Sensing.
Xiong, Hejian; Lacin, Emre; Ouyang, Hui; Naik, Aditi; Xu, Xueqi; Xie, Chen; Youn, Jonghae; Wilson, Blake A; Kumar, Krutin; Kern, Tyler; Aisenberg, Erin; Kircher, Daniel; Li, Xiuying; Zasadzinski, Joseph A; Mateo, Celine; Kleinfeld, David; Hrabetova, Sabina; Slesinger, Paul A; Qin, Zhenpeng.
Afiliación
  • Xiong H; Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, USA.
  • Lacin E; Nash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029-5674, USA.
  • Ouyang H; Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, USA.
  • Naik A; Department of Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, NY 11203, USA.
  • Xu X; Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, USA.
  • Xie C; Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, USA.
  • Youn J; Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, USA.
  • Wilson BA; Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, USA.
  • Kumar K; School of Behavioral and Brain Sciences, The University of Texas at Dallas, Richardson, TX 75080, USA.
  • Kern T; Nash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029-5674, USA.
  • Aisenberg E; Nash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029-5674, USA.
  • Kircher D; Nash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029-5674, USA.
  • Li X; Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, USA.
  • Zasadzinski JA; Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA.
  • Mateo C; Department of Physics, University of California at San Diego, La Jolla, CA 92093, USA.
  • Kleinfeld D; Department of Physics, University of California at San Diego, La Jolla, CA 92093, USA.
  • Hrabetova S; Department of Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, NY 11203, USA.
  • Slesinger PA; Nash Family Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029-5674, USA.
  • Qin Z; Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, USA.
Angew Chem Int Ed Engl ; 61(34): e202206122, 2022 08 22.
Article en En | MEDLINE | ID: mdl-35723610
ABSTRACT
Neuropeptides are abundant signaling molecules in the central nervous system. Yet remarkably little is known about their spatiotemporal spread and biological activity. Here, we developed an integrated optical approach using Plasmonic nAnovesicles and cell-based neurotransmitter fluorescent engineered reporter (CNiFER), or PACE, to probe neuropeptide signaling in the mouse neocortex. Small volumes (fL to pL) of exogenously supplied somatostatin-14 (SST) can be rapidly released under near-infrared light stimulation from nanovesicles implanted in the brain and detected by SST2 CNiFERs with nM sensitivity. Our measurements reveal reduced but synchronized SST transmission within 130 µm, and markedly smaller and delayed transmission at longer distances. These measurements enabled a quantitative estimation of the SST loss rate due to peptide degradation and binding. PACE offers a new tool for determining the spatiotemporal scales of neuropeptide volume transmission and signaling in the brain.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neuropéptidos Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neuropéptidos Límite: Animals Idioma: En Año: 2022 Tipo del documento: Article