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Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers.
Holmes, Susannah; Kirkwood, Henry J; Bean, Richard; Giewekemeyer, Klaus; Martin, Andrew V; Hadian-Jazi, Marjan; Wiedorn, Max O; Oberthür, Dominik; Marman, Hugh; Adriano, Luigi; Al-Qudami, Nasser; Bajt, Sasa; Barák, Imrich; Bari, Sadia; Bielecki, Johan; Brockhauser, Sandor; Coleman, Mathew A; Cruz-Mazo, Francisco; Danilevski, Cyril; Dörner, Katerina; Gañán-Calvo, Alfonso M; Graceffa, Rita; Fanghor, Hans; Heymann, Michael; Frank, Matthias; Kaukher, Alexander; Kim, Yoonhee; Kobe, Bostjan; Knoska, Juraj; Laurus, Torsten; Letrun, Romain; Maia, Luis; Messerschmidt, Marc; Metz, Markus; Michelat, Thomas; Mills, Grant; Molodtsov, Serguei; Monteiro, Diana C F; Morgan, Andrew J; Münnich, Astrid; Peña Murillo, Gisel E; Previtali, Gianpietro; Round, Adam; Sato, Tokushi; Schubert, Robin; Schulz, Joachim; Shelby, Megan; Seuring, Carolin; Sellberg, Jonas A; Sikorski, Marcin.
Afiliación
  • Holmes S; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.
  • Kirkwood HJ; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.
  • Bean R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Giewekemeyer K; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Martin AV; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Hadian-Jazi M; School of Science, RMIT University, Melbourne, VIC, 3000, Australia.
  • Wiedorn MO; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.
  • Oberthür D; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Marman H; Australian Nuclear Science and Technology Organisation (ANSTO), Sydney, NSW, 2234, Australia.
  • Adriano L; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
  • Al-Qudami N; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
  • Bajt S; Department of Mathematical and Physical Sciences, School of Engineering, Computing and Mathematical Sciences, La Trobe University, Melbourne, VIC, 3086, Australia.
  • Barák I; La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.
  • Bari S; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
  • Bielecki J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Brockhauser S; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
  • Coleman MA; The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany.
  • Cruz-Mazo F; Institute of Molecular Biology, SAS, Dubravska cesta 21, 845 51, Bratislava, Slovakia.
  • Danilevski C; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
  • Dörner K; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Gañán-Calvo AM; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Graceffa R; Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.
  • Fanghor H; Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092, Sevilla, Spain.
  • Heymann M; Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, 08544, USA.
  • Frank M; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Kaukher A; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Kim Y; Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092, Sevilla, Spain.
  • Kobe B; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Knoska J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Laurus T; Max-Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 175, 22761, Hamburg, Germany.
  • Letrun R; University of Southampton, Southampton, SO17 1BJ, UK.
  • Maia L; Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Am Pfaffenwaldring 57, 70569, Stuttgart, Germany.
  • Messerschmidt M; Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA, 94550, USA.
  • Metz M; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Michelat T; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Mills G; School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, QLD, 4072, Australia.
  • Molodtsov S; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
  • Monteiro DCF; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Morgan AJ; Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
  • Münnich A; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Peña Murillo GE; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Previtali G; School of Molecular Science, Arizona State University, Tempe, AZ, 85281, USA.
  • Round A; Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr 85, 22607, Hamburg, Germany.
  • Sato T; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Schubert R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Schulz J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Shelby M; Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger, Str. 23, 09599, Freiberg, Germany.
  • Seuring C; ITMO University, Kronverksky pr. 49, St. Petersburg, 197101, Russia.
  • Sellberg JA; The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, Hamburg, 22761, Germany.
  • Sikorski M; Hauptman-Woodward Medical Research Institute, 700 Ellicott St., Buffalo, NY, 14203, USA.
Nat Commun ; 13(1): 4708, 2022 08 11.
Article en En | MEDLINE | ID: mdl-35953469
ABSTRACT
The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Electrones / Rayos Láser Tipo de estudio: Diagnostic_studies Idioma: En Año: 2022 Tipo del documento: Article
Texto completo
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XML
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Electrones / Rayos Láser Tipo de estudio: Diagnostic_studies Idioma: En Año: 2022 Tipo del documento: Article