Role of Lactiplantibacillus plantarum strain RD1 (Lpb RD1) in mitochondria-mediated apoptosis: an in vitro analysis.
Arch Microbiol
; 204(9): 593, 2022 Sep 02.
Article
en En
| MEDLINE
| ID: mdl-36053319
ABSTRACT
The purpose of this study was to determine the cytotoxicity of Lactiplantibacillus plantarum strain RD1 (Lpb RD1), which was isolated and identified from the curd by 16 S rRNA sequencing. The probiotic properties of the isolated strain were studied by bile and NaCl tolerance and the ethyl acetate extract of Ea-LpRD1, was used to determine the toxicity against human breast cancer (MCF-7) cell lines and human embryonic kidney (HEK-293) cell lines by MTT assay. DNA fragmentation assay was carried out to study apoptosis induction. Flow cytometry analysis was done to determine the % of a cell population using the FTIC-Annexin V staining method. RT-PCR was used to assess gene expression levels in both cell lines. The IC50 concentration of the Ea-LpRD1 in MCF-7 cells was 0.30 mg/ml and in HEK-293 was 0.47 mg/ml. The expression levels of the BCL-2 gene anti-apoptotic genes in humans were reduced and BAX, caspase-8, caspase-3, and caspase-9 were an increased expression in MCF-7 cell lines.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Apoptosis
/
Mitocondrias
Límite:
Humans
Idioma:
En
Año:
2022
Tipo del documento:
Article