Enzyme-Linked Oligonucleotide Assay (ELONA).
Methods Mol Biol
; 2570: 235-242, 2023.
Article
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| MEDLINE
| ID: mdl-36156787
ABSTRACT
Aptamers are single-stranded oligonucleotides able to recognize a target with high affinity and specificity. Aptamers are used in different diagnostics applications, highlighting, among all, variations of the traditional enzyme-linked immunosorbent assay (ELISA). In this chapter, we show the procedures for the development of two types of indirect ELONA a sandwich ELONA and a direct ELONA coupled to either real-time quantitative PCR as a direct and sensitive readout.
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Banco de datos:
MEDLINE
Asunto principal:
Aptámeros de Nucleótidos
Idioma:
En
Año:
2023
Tipo del documento:
Article