Structural insights into the rational design of a nanobody that binds with high affinity to the SARS-CoV-2 spike variant.

Yamaguchi, Keishi; Anzai, Itsuki; Maeda, Ryota; Moriguchi, Maiko; Watanabe, Tokiko; Imura, Akihiro; Takaori-Kondo, Akifumi; Inoue, Tsuyoshi

Yamaguchi, Keishi; Anzai, Itsuki; Maeda, Ryota; Moriguchi, Maiko; Watanabe, Tokiko; Imura, Akihiro; Takaori-Kondo, Akifumi; Inoue, Tsuyoshi.

Afiliación

Yamaguchi K; Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
Anzai I; Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Maeda R; COGNANO Inc., 64-101 Kamitakano Higashiyama, Sakyo-ku, Kyoto, 601-1255, Japan.
Moriguchi M; Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.
Watanabe T; Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Imura A; COGNANO Inc., 64-101 Kamitakano Higashiyama, Sakyo-ku, Kyoto, 601-1255, Japan.
Takaori-Kondo A; Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
Inoue T; Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan.

J Biochem ; 173(2): 115-127, 2023 Feb 03.

Article en En | MEDLINE | ID: mdl-36413757

Asunto(s)

COVID-19; SARS-CoV-2; Humanos; SARS-CoV-2/genética; Dimerización; Epítopos; Cadenas Pesadas de Inmunoglobulina

Palabras clave

Crystal structure Abbreviations: ACE2, angiotensin converting enzyme 2; BLI, biolayer interferometry; CDR, complementarity-determining region; COVID-19, coronavirus disease 2019; cryo-EM, cryo-electron microscopy; EDTA, ethylenediaminetetraacetic acid; IPTG, Isopropyl ß-d-1-thiogalactopyranoside; mAb, monoclonal antibody; PBS, phosphate-buffered saline; RBD, receptor binding domain; SARS-CoV-2, severe acute respiratory syndrome coronavirus-2; VdW, Van der Waals; VHH, variable domain of heavy chain of heavy chain antibody; VOC, variants of concern; SARS-CoV-2; VHH; recognition mechanism

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Año: 2023 Tipo del documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google