Microwave-assisted and chemically tailored chlorogenic acid-functionalized silver nanoparticles of Citrus sinensis in gel matrix aiding QbD design for the treatment of acne.

ABSTRACT

BACKGROUND: Numerous studies have shown that various products of Citrus sinensis, for example, crude extracts, essential oil, and purified components, possess anti-acne properties. However, the development of chlorogenic acid-functionalized silver nanoparticles of C. sinensis in gel matrix aiding QbD design has not been evaluated for acne treatment. AIM: In this study, we have developed chlorogenic acid-functionalized silver nanoparticles of C. sinensis in a gel matrix employing a QbD approach for acne treatment. MATERIAL AND METHOD: Citrus sinensis extract-loaded silver nanoparticles were functionalized with chlorogenic acid, which acted as a bio-reducing agent and further improved anti-acne properties. The developed formulation was optimized via Box-Behnken Design. The formulation morphology was evaluated by transmission electron microscopy (TEM). The release profile of C. sinensis extract formulation was assessed by an in vitro release study and confocal laser scanning microscopy (CLSM). Moreover, the characterization study of the gel was performed that included an evaluation of the extrudability and spreadability of the developed gel. Furthermore, the anti-oxidant efficacy of AgN-CA formulation was validated using the DPPH test. RESULTS: The results showed a particle size of 71.78 nm, a polydispersity index of 0.297, a zeta potential of -36.12 mV, and an entrapment efficiency of 79.42% ± 6.79%. The results also indicated a uniform particle size. The release profile of C. sinensis extract revealed that 73.95% of the drug was released, whereas CLSM pictures of mice skin evidently demonstrated that the rhodamine B-treated AgN-CA gel penetrated much more extensively in comparison to the control. Furthermore, the anti-oxidant efficacy of AgN-CA formulation was validated using the DPPH test. Moreover, the characterization study of the developed gel, including its extrudability and spreadability, was found to be 16.02 ± 3.21 g and 30.73 ± 5.94 g cm/s, respectively. Also, texture analysis findings revealed that AgN-CA gel had firmness, consistency, cohesiveness, and viscosity index of 159.69 g, 634.95 g s, -116.33 g, and -501.80 g s, respectively, indicating that the AgN-CA gel was stable. CONCLUSION: The current study showed that AgN-CA is an effective drug carrier system for the topical administration of C. sinensis extract for the treatment of acne.