Microfluidic Impedance Cytometry for Single-Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae.
Glob Chall
; 7(3): 2200151, 2023 Mar.
Article
en En
| MEDLINE
| ID: mdl-36910468
ABSTRACT
Calcifying algae, like coccolithophores, greatly contribute to the oceanic carbon cycle and are therefore of particular interest for ocean carbon models. They play a key role in two processes that are important for the effective CO2 flux The organic carbon pump (photosynthesis) and the inorganic carbon pump (calcification). The relative contribution of calcification and photosynthesis can be measured in algae by the amount of particulate inorganic carbon (PIC) and particulate organic carbon (POC). A microfluidic impedance cytometer is presented, enabling non-invasive and high-throughput assessment of the calcification state of single coccolithophore cells. Gradual modification of the exoskeleton by acidification results in a strong linear fit (R 2 = 0.98) between the average electrical phase and the PICPOC ratio of the coccolithophore Emiliania huxleyi 920/9. The effect of different CO2 treatments on the PICPOC ratio, however, is inconclusive, indicating that there is no strong effect observed for this particular strain. Lower PICPOC ratios in cultures that grew to higher cell densities are found, which are also recorded with the impedance-based PICPOC sensor. The development of this new quantification tool for small volumes paves the way for high-throughput analysis while applying multi-variable environmental stressors to support projections of the future marine carbon cycle.
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Banco de datos:
MEDLINE
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2023
Tipo del documento:
Article