Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles.
Biophys J
; 122(15): 3078-3088, 2023 08 08.
Article
en En
| MEDLINE
| ID: mdl-37340636
ABSTRACT
A critical quality attribute for liquid formulations is the absence of visible particles. Such particles may form upon polysorbate hydrolysis resulting in release of free fatty acids into solution followed by precipitation. Strategies to avoid this effect are of major interest for the pharmaceutical industry. In this context, we investigated the structural organization of polysorbate micelles alone and upon addition of the fatty acid myristic acid (MA) by small-angle x-ray scattering. Two complementary approaches using a model of polydisperse core-shell ellipsoidal micelles and an ensemble of quasiatomistic micelle structures gave consistent results well describing the experimental data. The small-angle x-ray scattering data reveal polydisperse mixtures of ellipsoidal micelles containing about 22-35 molecules per micelle. The addition of MA at concentrations up to 100 µg/mL reveals only marginal effects on the scattering data. At the same time, addition of high amounts of MA (>500 µg/mL) increases the average sizes of the micelles indicating that MA penetrates into the surfactant micelles. These results together with molecular modeling shed light on the polysorbate contribution to fatty acid solubilization preventing or delaying fatty acid particle formation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Polisorbatos
/
Ácidos Grasos no Esterificados
/
Micelas
Idioma:
En
Año:
2023
Tipo del documento:
Article