Eye-Mimicked Neural Network Composed of Photosensitive Neural Spheroids with Human Opsin Proteins.
Adv Mater
; 35(35): e2302996, 2023 Sep.
Article
en En
| MEDLINE
| ID: mdl-37377148
ABSTRACT
An in vitro model, composed of the short-wavelength human opsins and rhodopsins, is created. Two types of photosensitive neural spheroids are transfected for selective reaction under bluish-purple and green lights. These are employed to two devices with intact neuron and neural-spheroid to study the interaction. By photostimulation, the photosensitive spheroid initiated photoactivation, and the signal generated from its body is transmitted to adjacent neural networks. Specifically, the signal traveled through the axon bundle in narrow gap from photosensitive spheroid to intact spheroid as an eye-to-brain model including optic nerve. The whole process with photosensitive spheroid is monitored by calcium ion detecting fluorescence images. The results of this study can be applied to examine vision restoration and novel photosensitive biological systems with spectral sensitivity.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Visión Ocular
/
Opsinas
Límite:
Humans
Idioma:
En
Año:
2023
Tipo del documento:
Article