Tissue Washing Improves Native Ambient Mass Spectrometry Detection of Membrane Proteins Directly from Tissue.
J Am Chem Soc
; 145(29): 15658-15662, 2023 07 26.
Article
en En
| MEDLINE
| ID: mdl-37459360
ABSTRACT
Native ambient mass spectrometry enables the in situ analysis of proteins and their complexes directly from tissue, providing both structural and spatial information. Until recently, the approach was applied exclusively to the analysis of soluble proteins; however, there is a drive for new techniques that enable analysis of membrane proteins. Here we demonstrate native ambient mass spectrometry of membrane proteins, including ß-barrel and α-helical (single and multipass) integral membrane proteins and membrane-associated proteins incorporating lipid anchors, by integration of a simple washing protocol to remove soluble proteins. Mass spectrometry imaging revealed that washing did not disrupt the spatial distributions of the membrane and membrane-associated proteins. Some delocalization of the remaining soluble proteins was observed.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de la Membrana
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2023
Tipo del documento:
Article